| 小鼠胚胎干细胞的培养 |
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| 引用本文： | 赵玲,杜晓兰,王建民,陈林.小鼠胚胎干细胞的培养[J].现代生物医学进展,2007,7(6):916-918. |
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| 作者姓名： | 赵玲 杜晓兰 王建民 陈林 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所全军创伤中心,创伤、烧伤与复合伤国家重点实验室,重庆,400042 |
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| 基金项目： | 国家自然科学基金;国家自然科学基金 |
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| 摘 要： | 目的:建立小鼠胚胎干细胞(embryonic stem cells,ES)的培养方法。方法:制备G418抗性的原代小鼠胚胎成纤维细胞,经丝裂霉素C处理后成滋养层细胞,将小鼠胚胎干细胞复苏后,应用含白血病抑制因子的ES细胞培养液,培养小鼠ES细胞,观察集落的生长情况,并在光镜下观察细胞形态。结果:小鼠胚胎成纤维细胞生长良好,ES细胞呈克隆状生长,且保持未分化状态。结论:建立了小鼠胚胎干细胞培养的有效方法,为下一步基因打靶奠定基础。
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| 关 键 词： | 胚胎干细胞 小鼠 培养 |
| 文章编号： | 24517585 |
| 修稿时间： | 03 1 2007 12:00AM |
Culture of Mouse Embryonic Stem Cells |
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| ZHAO Ling,DU Xiao-lan,WANG Jian-min,CHEN Lin.Culture of Mouse Embryonic Stem Cells[J].Progress in Modern Biomedicine,2007,7(6):916-918. |
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| Authors: | ZHAO Ling DU Xiao-lan WANG Jian-min CHEN Lin |
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| Institution: | Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University; State Key Laboratory of Trauma, Bums and Combined Injury, Chongqing 400042 |
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| Abstract: | Objective:To establish mouse embryonic stem cells culture system and methods.Methods:Mitomycin G- treated G418-resistant mouse embryonic fibroblasts(MEFs)were used as feeder layer cells.The mouse embryonic stem cells(ES cells)were co-cultured with MEFs in media of DMEM supplement with leukemia inhibitory factor(LIF).Results:The MEFs grew well and the ES cell colonies grew in small dusters and remain undifferentiated.Conclusions:The culture system of mouse ES cells was established,which will provide the possibility for obtaining gene targeted ES cells. |
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| Keywords: | Embryonic stem cell Mice Culture |
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