TGF—β receptors in mouse ES—5 cells and their differentiated derivatives |
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引用本文: | SHIWEIKANG JUNWU 等.TGF—β receptors in mouse ES—5 cells and their differentiated derivatives[J].细胞研究,1995,5(1):35-45. |
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作者姓名: | SHIWEIKANG JUNWU |
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作者单位: | [1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031,China [2]ShanghaiInstituteofCellB,ChineseAcademyofSciences,Shanghai200031,China |
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摘 要: | By radioreceptor binding studies with iodinated TGF-β1,it has been shown that an undifferentiated ES-5 cell expresses approximately 3270 receptors with a dissociation constant Kd-130pM,but after the induction of differentiation by retinoic acid and dBcAMP,the receptor number of a differentiated RA-ES-5 cell was increased about 80% and the Kd was also increased to 370 pM.Furthermore,more direct evidence supporting the expression of TGF-β type I and type Ⅱ receptors in both ES-5 and RA-ES-5 cells has come from dot blot hybridization of cellular mRNA with cDNA probes for type I and type Ⅱ receptors.Meanwhile,mRNA expression level of types I and Ⅱ receptors in R-ES-5 cells were higher than that in ES-5 cells.Down-regulation of TGF-β receptors with a significant decrease in the rate of cell proliferation in both cells,was found by employing a pretreatment with neutralizing antibody to TGF-β1.The possible role of receptors for TGF-β in cell differentiation is discussed here.
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关 键 词: | TGF-β受体 鼠ES-5细胞 RA诱导分化 转化生长因子-β 中和抗体 下向调节 胚胎干细胞 |
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