Characterization of ribonucleolytic activity of angiogenin towards tRNA |
| |
| Authors: | F S Lee B L Vallee |
| |
| Affiliation: | Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115. |
| |
| Abstract: | Yeast tRNA is a convenient substrate for the assay of the ribonucleolytic activity of human angiogenin. The optimal pH, [NaCl], and temperature for tRNA cleavage by angiogenin are approximately 6.8, 15-30 mM, and approximately 55 degrees C, respectively, as compared with approximately 8.0, 100-200 mM, and approximately 65 degrees C, respectively, for RNase A. Polyanions and metals both inhibit angiogenin and RNase A but to different extents. |
| |
| Keywords: | |
|
|
