Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila |
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| Authors: | Ida Takanori Takahashi Tomoko Tominaga Hatsumi Sato Takahiro Kume Kazuhiko Ozaki Mamiko Hiraguchi Tetsutaro Maeda Toru Shiotani Hajime Terajima Saki Sano Hiroko Mori Kenji Yoshida Morikatsu Miyazato Mikiya Kato Johji Murakami Noboru Kangawa Kenji Kojima Masayasu |
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| Affiliation: | aInterdisciplinary Research Organization, University of Miyazaki, Miyazaki 889-1692, Japan;bDepartment of Biology, School of Medicine, Kurume University, Fukuoka 839-0864, Japan;cMolecular Genetics, Institute of Life Sciences, Kurume University, Fukuoka 839-0864, Japan;dDepartment of Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan;eDepartment of Biology, Graduate School of Science, Kobe University, Hyogo 657-8501, Japan;fOchadai Academic Production, Division of Advanced Sciences, Ochanomizu University, Tokyo 112-8610, Japan;gDepartment of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Osaka 565-8565, Japan;hFrontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan;iDepartment of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2155, Japan |
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| Abstract: | A number of bioactive peptides are involved in regulating a wide range of animal behaviors, including food consumption. Vertebrate neuropeptide Y (NPY) is a potent stimulator of appetitive behavior. Recently, Drosophila neuropeptide F (dNPF) and short NPF (sNPF), the Drosophila homologs of the vertebrate NPY, were identified to characterize the functions of NPFs in the feeding behaviors of this insect. Dm-NPFR1 and NPFR76F are the receptors for dNPF and sNPF, respectively; both receptors are G protein-coupled receptors (GPCRs). Another GPCR (CG5811; NepYR) was indentified in Drosophila as a neuropeptide Y-like receptor. Here, we identified 2 ligands of CG5811, dRYamide-1 and dRYamide-2. Both peptides are derived from the same precursor (CG40733) and have no significant structural similarities to known bioactive peptides. The C-terminal sequence RYamide of dRYamides is identical to that of NPY family peptides; on the other hand, dNPF and sNPF have C-terminal RFamide. When administered to blowflies, dRYamide-1 suppressed feeding motivation. We propose that dRYamides are related to the NPY family in vertebrates, similar to dNPF and sNPF. |
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| Keywords: | GPCR Novel bioactive peptide Drosophila Neuropeptide Y |
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