Sodium fluoride induces apoptosis and alters bcl-2 family protein expression in MC3T3-E1 osteoblastic cells |
| |
Authors: | Yang Shiyong Wang Zhi Farquharson Colin Alkasir Rashad Zahra Mohammad Ren Gaixian Han Bo |
| |
Institution: | aCollege of Veterinary Medicine, China Agricultural University, 100193 Beijing, China;bThe Roslin Institute, R(D)SVS, University of Edinburgh, EH25 9PS, Scotland, UK |
| |
Abstract: | Chronic excessive fluoride intake is known to be toxic and can lead to fluorosis and bone pathologies. However, the cellular mechanisms underlying NaF-induced cytotoxicity in osteoblasts are not well understood. The objectives of this study were to determine the effects of fluoride treatment on MC3T3-E1 osteoblastic cell viability, cell cycle analysis, apoptosis and the expression levels of bcl-2 family members: bcl-2 and bax. MC3T3-E1 cells were treated with 10−5; 5 × 10−5; 10−4; 5 × 10−4 and 10−3 M NaF for up to 48 h. NaF was found to reduce cell viability in a temporal and concentration dependent manner and promote apoptosis even at low concentrations (10−5 M). This increased apoptosis was due to alterations in the expression of both pro-apoptotic bax and anti-apoptotic bcl-2. The net result was a decrease in the bcl-2/bax ratio which was found at both the mRNA and protein levels. Furthermore, we also noted that NaF-induced S-phase arrest during the cell cycle of MC3T3-E1 cells. These data suggest that fluoride-induced osteoblast apoptosis is mediated by direct effects of fluoride on the expression of bcl-2 family members. |
| |
Keywords: | Fluoride Apoptosis Bcl-2 family proteins MC3T3-E1 osteoblastic cells |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|