| 肺炎支原体黏附蛋白P30的高效表达及初步应用 |
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| 引用本文： | 王成红,;于传亭,;张奇舒,;李鹏飞,;王晓丹,;修冰水,;张贺秋. 肺炎支原体黏附蛋白P30的高效表达及初步应用[J]. 生物技术通讯, 2014, 0(6): 821-823 |
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| 作者姓名： | 王成红, 于传亭, 张奇舒, 李鹏飞, 王晓丹, 修冰水, 张贺秋 |
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| 作者单位： | [1]烟台市烟台山医院检验科,山东烟台264000; [2]军事医学科学院基础医学研究所,北京100850 |
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| 基金项目： | 国家重大科技专项（2013ZX10004803） |
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| 摘 要： |
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| 关 键 词： | 肺炎支原体 P30黏附蛋白 优势密码子 原核表达 |
Expression of P30 Protein of Mycoplasma pneumoniae in Escherichia coli |
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| Affiliation: | WANG Cheng-Hong, YU Chuan-Ting, ZHANG Qi-Shu, LI Peng-Fei, WANG Xiao-Dan, XIU Bing-Shui, ZHANG He-Qiu (1. Yantai Mountain Hospital Clinical Laboratory, Yantai 264000; 2. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850; China) |
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| Abstract: | Objective： To obtain recombinant P30 adhesion protein of Mycoplasma pneumoniae（Mp） for the study of Mp diagnostic reagents and vaccines. Methods： The predominant epitopes of P30 protein were selected by bioin？formatic analysis and the DNA sequences were designed according to of high-usage codons of E.coli. Above gene was expressed in E.coli by vector pBVIL1, and its immunoreactivity was measured by ELISA. Results： The codon adaptation index（CAI） of optimized P30 gene was 0.84, which was higher than that of wild gene. The molecular weight of IL1-P30 fusion protein expressed in E.coli was 43.5 kD. The sensitivity and specificity of ELISA based on P30 were 82.35% and 89.36% respectively. The AUC of the ELISA was 0.9088 according to ROC analysis. Conclusion： The P30 of Mp was expressed in E.coli by codon optimization, which provides an important reference for the study of diagnostic reagents of Mp infection. |
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| Keywords: | Mycoplasma pneumoniae P30 adhesion protein codon optimization prokaryotic expression |
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