人脐带间充质干细胞对百草枯中毒肺损伤小鼠模型的治疗作用

目的:探讨脐带间充质干细胞(UCMSC)治疗百草枯中毒引起的小鼠肺损伤的可行性。方法:小鼠腹腔一次性注射百草枯制备百草枯中毒小鼠模型,24 h后尾静脉注射UCMSC,分别于治疗后7和21 d取材,观察UCMSC对急性肺损伤和慢性肺纤维化的治疗作用。结果:UCMSC移植对40和50 mg/kg百草枯染毒组急性肺损伤有效,动物死亡率显著降低,但对60 mg/kg百草枯染毒组动物无效。UCMSC治疗对慢性肺纤维化有显著治疗作用,治疗组动物体重恢复早,死亡率降低,肺纤维化评分降低。RT-PCR结果显示,UCMSC移植3 h有人特异性线粒体基因的表达,但24 h后未检测到。结论:UCMSC对百草枯中毒性急慢性肺损伤有一定的治疗作用,这种作用可能是通过旁分泌机制实现的。

Therapeutic Effect of Human Umbilical Cord Mesenchymal Stem Cells on Paraquat-Induced Lung Injury in Mouse

Objective： To investigate the possibility if human umbilical cord mesenchymal stem cells（UCMSC） can attenuate paraquat-induced lung injury in mouse. Methods： Mice received one dose of intraperitoneal injec？tion of paraquat to prepare animal models of lung injury. UCMSC were injected via tail vein 24 hours later. Then, the animals were executed 7 or 21 days later to evaluate therapeutic effect of cell treatment on the acute lung injury and lung fibrosis. Results： UCMSC decreased the death rates of 40 and 50 mg/kg paraquat intake groups, but had no effect on the animals in 60 mg/kg group. And the cell treatment also ameliorated the score of lung fibrosis and the body weight of the animals recovered earlier than control group. The RT-PCR result of hu？man specific chrome B mitochondria showed that human cells existed in the lung after 3 hours of transplantation, but didn＆#39;t been detected 24 hours later. Conclusion： These results showed that UCMSC have therapeutic effect on acute lung injury and lung fibrosis induced by paraquat poisoning. And this effect may be due to the paracrine characters of mesenchymal stem cells.