Growth factor regulation of human growth plate chondrocyte proliferation in vitro |
| |
Authors: | Olney Robert C Wang Jianwei Sylvester James E Mougey Edward B |
| |
Affiliation: | Cellular and Molecular Medicine Laboratory, The Nemours Children's Clinic, 807 Children's Way, Jacksonville, FL 32207, USA. rolney@nemours.org |
| |
Abstract: | Linear growth occurs as the result of growth plate chondrocytes undergoing proliferative and hypertrophic phases. Paracrine feedback loops that regulate the entry of chondrocytes into the hypertrophic phase have been shown and similar pathways likely exist for the proliferative phase. Human long-bone growth plate chondrocytes were cultured in vitro. The proliferative effects of a variety of factors were determined by [3H]thymidine uptake and the gene expression profile of these cells was determined by DNA microarray analysis. Serum, insulin-like growth factor (IGF)-I and -II, transforming growth factor-beta (TGF-beta, fibroblast growth factor (FGF)-1, -2, and -18, and platelet-derived growth factor (PDGF)-BB were potent stimulators of proliferation. FGF-10, testosterone, and bone morphogenetic proteins (BMP)-2, -4, and -6 inhibited proliferation. Microarray analysis showed that the genes for multiple members of the IGF-I, TGF-beta, FGF, and BMP pathways were expressed, suggesting the presence of autocrine/paracrine pathways that regulate the proliferative phase of growth plate-mediated growth. |
| |
Keywords: | Chondrocytes Growth plate Insulin-like growth factors Transforming growth factor-β Fibroblast growth factors Bone morphogenetic proteins Microarray analysis Cell division |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|