Dopamine sulfate formation and phenol sulfotransferase activity in dog and human platelets |
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| Authors: | L A Toth M C Scott M A Elchisak |
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| Institution: | 1. Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari “Aldo Moro”, Via E. Orabona, 4, I-70125 Bari, Italy;2. Dipartimento di Scienze del Farmaco, Università degli Studi di Pavia, viale Taramelli, 12, 27100 Pavia, Italy;1. Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan;2. State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Biology, School of Life Sciences, Xiamen University, Xiamen, China;1. Pharmaceutical Science and CMC Biologics, H. Lundbeck A/S, Ottiliavej 9, DK-2500 Valby, Denmark;2. NSM, Research Unit for Functional Biomaterials, Roskilde University, Universitetsvej 1, DK-4000 Roskilde, Denmark;1. Department of Veterinary Surgery, Faculty of Veterinary Medicine, Tokyo University of Agriculture and Technology, Saiwaicho 3-5-8, Fuchu, Tokyo 183-8509, Japan;2. Veterinary Diagnostic Imaging, Faculty of Veterinary Medicine, Tokyo University of Agriculture and Technology, Saiwaicho 3-5-8, Fuchu, Tokyo 183-8509, Japan |
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| Abstract: | High performance liquid chromatography with radioactive flow detection was used to examine the accumulation and sulfoconjugation of dopamine by human and dog platelets. Platelets from both species accumulated similar amounts of dopamine from the incubation medium, but only human platelets were found to convert 3H-dopamine to 3-H-dopamine sulfate. This difference between the two species was associated with a relative absence of phenol sulfotransferase activity in dog platelets as compared to human platelets. Dog platelets did not appear to contain an inhibitor of phenol sulfotransferase activity. Despite the apparent difference in the ability of platelets to form dopamine sulfate, conc concentrations of dopamine-3-O-sulfate and dopamine-4-O-sulfate were similar in dog and human plasma. These data suggest that platelets may represent a potential source of at least some of the dopamine sulfate found in human plasma, but not in dog plasma. |
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