Molecular cloning and nucleotide sequence of the pyruvate kinase gene of an actinomycete Microbispora thermodiastatica |
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Authors: | A Arai S Masuda A Matsuyama S Murakami M Nakajima |
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Institution: | (1) Research and Development Division, Kikkoman Corporation, 399 Noda, Noda-shi, Chiba, 278, Japan Tel.: +81 471 23 5528 Fax: +81 471 23 5550, JP |
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Abstract: | The gene for the thermostable pyruvate kinase of Microbispora thermodiastatica IFO 14046, a moderate thermophilic actinomycete, was cloned in Escherichia coli. This gene consists of an open reading frame of 1422 nucleotides and encodes a protein of 474 amino acids with molecular
mass of 50 805 Da. The open reading frame was confirmed as the pyruvate kinase gene by comparison with the N-terminal amino
acid sequence of the purified pyruvate kinase from M. thermodiastatica.
Received: 19 May 1997 / Received last revision: 22 September 1997 / Accepted: 14 October 1997 |
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