Qualitative and quantitative analysis of serum immunoglobulins of four Antarctic fish species |
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| Authors: | G Scapigliati F Chausson E L Cooper D Scalia M Mazzini |
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| Institution: | (1) Dipartimento di Scienze Ambientali, Università della Tuscia, I-01100 Viterbo, Italy Fax +39-761-357114; e-mail: scapigg@unitus.it, IT;(2) Deparment of Anatomy and Cell Biology, University of California, Los Angeles, CA, USA , US |
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| Abstract: | Immunoglobulins from the Antarctic fish species Trematomus bernacchii, Trematomus hansoni, Trematomus newnesi, and Chionodraco hamatus were analysed in whole serum and after purification by affinity chromatography on protein A-sepharose. Using SDS-PAGE, the
apparent masses of the heavy and light chains were, respectively, 83.5 kDa and 27.5 kDa for T. bernacchii, 83.5 kDa and 27 kDa for T. hansoni, 81 kDa and 27.5 kDa for T. newnesi, and 74.5 kDa and 30 kDa for C. hamatus. It was not possible to purify immunoglobulins from T. newnesi due to their low concentration in serum. Heterogeneity in mass of both heavy and light chains was observed in all species.
By using a polyclonal antibody raised against sea bass immunoglobulins, cross-reactivity was observed with heavy and light
chains of all species. With this antibody, an indirect enzyme-linked immunosorbent assay (ELISA) was developed and results
showed the relative immunoglobulin concentration in sera of the Antarctic fish species considered, using as standard sea bass
immunoglobulins.
Received: 25 November 1996 / Accepted: 3 February 1997 |
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