Isolation and Characterization of a Water Stress-Specific Genomic Gene, pwsi 18, from Rice |
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Authors: | Joshee, Nirmal Kisaka, Hiroaki Kitagawa, Yoshichika |
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Affiliation: | 1 Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agriculture Ogata, Akita, 010-04 Japan 2 Institute of Genetic Ecology, Tohoku University Sendai, 980-77 Japan |
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Abstract: | One of the water stress-specific cDNA clones of rice characterisedpreviously, wsi18, was selected for further study. The wsi18gene can be induced by water stress conditions such as mannitol,NaCl, and dryness, but not by ABA, cold, or heat. A genomicclone for wsi18, pwsi18, contained about 1.7 kbp of the 5' upstreamsequence, two introns, and the full coding sequence. The 5'-upstreamsequence of pwsi18 contained putative cis-acting elements, namelyan ABA-responsive element (ABRE), three G-boxes, three E-boxes,a MEF-2 sequence, four direct and two inverted repeats, andfour sequences similar to DRE, which is involved in the dehydrationresponse of Arabi-dopsis genes. The gusA reporter gene underthe control of the pwsi18 promoter showed transient expressionin response to water stress. Deletion of the downstream DRE-likesequence between the distal G-boxes-2 and -3 resulted in ratherlow GUS expression. (Received March 27, 1997; Accepted November 5, 1997) |
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