Steroids,histamine, and serotonin in the medicinal leech salivary gland secretion |
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| Authors: | I. P. Baskova Z. Ferner A. S. Balkina S. A. Kozin O. V. Kharitonova L. L. Zavalova V. G. Zgoda |
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| Affiliation: | (1) Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia;(2) Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 119997 Moscow, Russia;(3) Faculty of Chemistry, Lomonosov Moscow State University, 119992 Moscow, Russia |
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| Abstract: | Lipids represent 20% of the total weight of the dried pool of medicinal leech salivary gland secretion (SGS) obtained from about 50 individual animals. SGS lacks phospholipids, but contains steroids. Immunochemiluminescent analysis of SGS revealed the presence of free steroid hormones: cortisol, progesterone, testosterone, estradiol, and dehydroepiandrosterone. Micro-chromatographic-mass spectrometric analysis of SGS and its low molecular weight fraction (LMW) (molecular masses ranged from 220 to 850 Da) has shown the multicomponent nature of the LMW fraction. Using standard preparations as the reference steroid hormones (cortisol, dehydroepiandrosterone, androstenedione, and testosterone) and histamine and serotonine have been identified in SGS. |
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| Keywords: | lipids steroid hormones histamine serotonin salivary gland secretion medicinal leech |
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