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A novel alkaline lipase from Ralstonia with potential application in biodiesel production |
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| Authors: | Yoo Hah-Young Simkhada Jaya Ram Cho Seung Sik Park Don Hee Kim Seung Wook Seong Chi Nam Yoo Jin Cheol |
| Affiliation: | a Department of Chemical and Biological Engineering, Korea University, Seoul, Republic of Korea b Department of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea c Interdisciplinary Program of Graduate School for Bioenergy and Biomaterials, Chonnam National University, Gwangju 500-757, Republic of Korea d Department of Biology, College of Natural Sciences, Sunchon National University, Sunchon 540-742, Republic of Korea |
| Abstract: | With the aim of isolating a biocatalyst able to catalyze biodiesel production from microbial source, Ralstonia sp. CS274 was isolated and a lipase from the strain (RL74) was purified. Molecular weight of RL74 was estimated to be 28,000 Da by SDS-PAGE. The activity was highest at 50-55 °C and pH 8.0-9.5 and was stable at pH 7.0-12.0 and up to 45 °C. It was resistant to oxidizing and reducing agents and the activity was enhanced by detergents. RL74 was 1,3 specific and Km and Vmax for p-nitrophenyl palmitate were 2.73 ± 0.6 mM and 101.4 ± 1.9 mM/min mg, respectively. N-terminal amino acid sequence showed partial homology with that of Penicillium lipases. RL74 produced biodiesel more efficiently in palm oil than in soybean oil; and the production was highest at pH 8.0, at 5% methanol and at 20% water content. |
| Keywords: | Ralstonia sp. CS274 Lipase Purification Biodiesel production |
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