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Modulating glycosidase degradation and lectin recognition of gold glyconanoparticles |
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| Authors: | Africa G Barrientos Jesús M de la Fuente Marta Jimnez Dolores Solís F Javier Caada Manuel Martín-Lomas Soledad Penads |
| Institution: | aLaboratory of Glyconanotechnology, IIQ-CSIC, Seville, Spain;bInstituto de Química Física Rocasolano, CSIC, Madrid, Spain;cCIBER de Enfermedades Respiratorias, Spain;dCentro de Investigaciones Biológicas, CSIC, Madrid, Spain;eCIC biomaGUNE/CIBER-BNN, Paseo Miramón 182, Parque Tecnológico de San Sebastián, 2009, San Sebastián, Guipúzcoa, Spain |
| Abstract: | Glyconanoparticles (GNPs) are water-soluble carbohydrate-functionalized gold nanoclusters with a promising potential to serve as versatile tools in studies ranging from basic chemical glycobiology to clinical applications. In this paper we evaluate the influence of ligand density and presentation on the recognition by protein receptors by examining the interaction of lactose-functionalized GNPs with two different galactose-specific carbohydrate-binding proteins: an enzyme, Escherichia coli β-galactosidase, and a lectin, Viscum album agglutinin. The results suggest that the proper selection of ligand densities and spacers in GNP functionalization is an important requisite to match the topological requirements of the target receptor while escaping glycosidase degradation. |
| Keywords: | Gold glyconanoparticles Glycosidases Lectins Multivalent presentation Molecular recognition |
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