Expression of heat shock protein 90 at the cell surface in human neuroblastoma cells |
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Authors: | Cristina Cid Ignacio Regidor Pedro D Poveda Alberto Alcazar |
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Institution: | (1) Center for Astrobiology, CSIC-INTA, Torrejón de Ardoz, Spain;(2) Departments of Neurophysiology and Neurosurgery, Hospital Ramón y Cajal, Madrid, Spain;(3) Department of Investigation, Hospital Ramón y Cajal, Ctra. Colmenar km 9.1, 28034 Madrid, Spain |
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Abstract: | In addition to the activity of heat shock protein 90 (Hsp90/HSPC) as a chaperone, some recent studies have reported expression
of Hsp90 at the cell surface in certain types of cancer and nervous system cells. We study the expression of Hsp90 at the
cell surface in human neuroblastoma (NB69) cells. Immunofluorescence experiments labeling with anti-Hsp90 antibodies on both
nonpermeabilized cells and live cells detected Hsp90 at the cell surface. Hsp90 was also identified in a membrane fraction
from subcellular fractionation. Cell-surface Hsp90 was significantly more expressed in undifferentiated proliferative spherical
neuroblastoma cells than in differentiated flattened cells. In addition, spherical cells were significantly more sensitive
to Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin compared to flattened cells. This paper describes the first evidence
of cell-surface Hsp90 expression in a cancer cell line from nervous tissue and may indicate a novel target for anti-tumoral
agents. |
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Keywords: | Hsp90 expression Cell surface Neuroblastoma Hsp90 inhibitors 17-allylamino-17-demethoxygeldanamycin |
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