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Characterization of two chlorophyll b-deficient,azide-derived mutants of Hordeum vulgare cv. Maris Mink
Authors:Janice C Turner  Mary L Parker  Stephen J Temple  Peter J Lea
Institution:(1) Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ Harpenden, Herts, UK;(2) Present address: Food Research Institute, Colney Lane, NR4 7UA Norwich, UK;(3) Present address: Department of Biological Sciences, University of Lancaster, LA1 4YQ Lancaster, UK
Abstract:Two mutant lines of Hordeum vulgare cv. Maris Mink (designated RChl 46 and 47) deficient in chlorophyll b have been isolated following azide mutagenesis. Two major thylakoid membrane proteins of molecular weight 25 and 26 k daltons are absent from the mutant plants following analysis by SDS gel electrophoresis, presumably due to a lack of the light harvesting chlorophyll a/b complex. The photosynthetic capabilities of the wild type and mutant lines were very similar.
Keywords:chlorophyll b deficiency  barley  Hordeum vulgare  mutant  thylakoid membrane proteins teins
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