The ROSA26 LacZ-neo R insertion confers resistance to mammary tumors in Apc Min/+ mice |
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Authors: | Rebecca L Kohlhepp Laura F Hegge and Amy R Moser |
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Institution: | (1) The Department of Human Oncology, K4/330 CSC 3864, 600 Highland Ave, University of Wisconsin-Madison, Madison, Wisconsin 53792, USA, US |
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Abstract: | B6.129S7-Gtrosa26 (ROSA26) mice carry a LacZ-neo
R
insertion on Chromosome (Chr) 6, made by promoter trapping with AB1 129 ES cells. Female C57BL/6J Apc
Min
/+ (B6 Min/+) mice are very susceptible to the induction of mammary tumors after treatment with ethylnitrosourea (ENU). However, ENU-treated
B6 mice carrying both Apc
Min
and ROSA26 are resistant to mammary tumor formation. Thus, ROSA26 mice carry a modifier of Min-induced mammary tumor susceptibility. We have previously mapped the modifier to a 4-cM interval of 129-derived DNA that also
contains the ROSA26 insertion. Here we report additional evidence for the effect of the ROSA26 insertion on mammary tumor
formation. To test the hypothesis that the resistance was due to a linked modifier locus, we utilized two approaches. We have
derived and tested two lines of mice that are congenic for 129-derived DNA within the minimal modifier interval and show that
they are as susceptible to mammary tumors as are B6 mice. Additionally, we analyzed a backcross population segregating for
the insertion and show that mice carrying the insertion are more resistant to mammary tumor development than are mice not
carrying the insertion. Thus, the resistance is not due to a 129-derived modifier allele, but must be due to the ROSA26 insertion.
In addition, the effect of the ROSA26 insertion can be detected in a backcross population segregating for other mammary modifiers.
Received: 29 December 2000 / Accepted: 4 April 2001 |
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Keywords: | |
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