Effect of protocatechuic acid from Alpinia oxyphylla on proliferation of human adipose tissue-derived stromal cells in vitro |
| |
Authors: | Han Wang Tian-Qing Liu Yan-Xia Zhu Shui Guan Xue-Hu Ma Zhan-Feng Cui |
| |
Affiliation: | 1. Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Environmental and Life Science Building 3-403, No. 2 Linggong Road, Ganjingzi District, Dalian, 116024, China 2. Department of Engineering Science, Oxford University, Oxford, OX1 3PJ, UK
|
| |
Abstract: | The effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-dependent manner, while no obvious effect of catapol on the proliferation of hADSCs was observed. In addition, the cell number of hADSCs treated by 1.5 mM PCA increased in a time-dependent manner. The flow cytometric analysis of DNA content demonstrated the cell cycle progress from the G0/G1 phase to the S phase. Western blot analysis revealed the elevated expression of cyclin D1 in hADSCs induced by PCA treatment. Cyclin D1-siRNA transfection significantly inhibit the promotion of cell proliferation by PCA. Furthermore, the flow cytometric analysis of the cell surface antigens and the multidifferential potential tests of PCA-treated hADSCs showed that the cells retained their functional characteristics of multipotential mesenchymal progenitors. It is concluded that PCA can effectively up-regulate the proliferation of hADSCs. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|