Total chromium in the human lens |
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Authors: | Alain Pineau Olivier Guillard Françoise Chauvelon Jean-François Risse |
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Institution: | 1. Laboratoire de Toxicologie, Faculté de Pharmacie, Nantes, France 2. Centre de Dosage des Eléments-Trace, Laboratoire de Biochimie, Poitiers, France 3. Service d'Ophtalmologie, H?pital Jean Bernard, Poitiers, France
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Abstract: | A simple, quick procedure has been developed for preparation of human lenses for total chromium (Cr) analysis by electrothermal
atomic absorption spectrometry (EAAS). This procedure involves wet ashing in a mini-autoclave with HNO3 and H2SO4. Recovery was 101.6%. This procedure is simple to carry out, does not generate corrosive fumes, and minimizes contaminations.
Measurements obtained by this method give values similar to those found previously by more cumbersome methods. It can be conveniently
used to prepare biological samples for ultratrace analysis. The mean Cr concentration in human lenses varied between 0.345±0.147
μg/g dry wt in the normal population and 0.205±0.160 μg/g dry wt in cataractous lenses. |
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Keywords: | Chromium human lenses electrothermal atomic absorption spectrometry Zeeman effect mineralization |
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