Genotypic control of peanut somatic embryogenesis |
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Authors: | K Chengalrayan V B Mhaske S Hazra |
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Institution: | (1) Plant Tissue Culture Division, National Chemical Laboratory, Pune - 411 008, India Fax: +91-0212-330233 e-mail: shazra@dalton.ncl.res.in, IN |
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Abstract: | The protocol for obtaining a high frequency of plant development via somatic embryogenesis from mature zygotic embryo-derived
leaflets of peanut (Arachis hypogaea L.) involves multiple stages; these include the induction of embryogenic masses, development of embryos, radicle emergence/conversion
of embryos and the development of plants from rooted abnormal embryos. Sixteen genotypes were subjected to this protocol by
exposing mature zygotic embryo-derived leaflets to the common media sequence and comparing responses. Although the protocol
was effective for all the genotypes, variation in frequency of response at each stage of development indicated that, with
the exception of root meristem differentiation and subsequent radicle emergence, the whole process of somatic embryogenesis
depended on the genotypic constitution of the original plant. The failure of somatic embryos to undergo conversion to plantlets
could be a genotype-dependent characteristic.
Received: 5 June 1997 / Revision received: 2 December 1997 / Accepted: 12 December 1997 |
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Keywords: | Arachis hypogaea L Somatic embryogenesis Genotypes |
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