Roles of PprA,IrrE, and RecA in the resistance of <Emphasis Type="Italic">Deinococcus radiodurans</Emphasis> to germicidal and environmentally relevant UV radiation |
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Authors: | Anja Bauermeister Esma Bentchikou Ralf Moeller Petra Rettberg |
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Institution: | 1.Institute of Aerospace Medicine, Radiation Biology Division, Research Group ‘Astrobiology’,German Aerospace Center (DLR),Cologne,Germany;2.Institut de Génétique et Microbiologie,Université Paris-Sud 11, CNRS UMR8621, CEA LRC 42 V,91405,Orsay,France |
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Abstract: | To study the role of different DNA repair genes in the resistance of Deinococcus radiodurans to mono- and polychromatic UV radiation, wild-type strain and knockout mutants in RecA, PprA, and IrrE of D. radiodurans were irradiated with UV-C (254 nm), UV-(A + B) (280–400 nm) and UV-A (315–400 nm) radiation, and survival was monitored.
The strain deficient in recA was highly sensitive to UV-C radiation compared to the wild-type, but showed no loss of resistance against irradiation with
UV-(A + B) and UV-A, while pprA and irrE-deficient strains exhibited elevated sensitivity to UV-A and UV-(A + B) radiation. These results suggest that the repair
of DNA double-strand breaks is essential after treatment with highly energetic UV-C radiation, whereas protection from oxidative
stress may play a greater role in resistance to environmentally relevant UV radiation. |
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