A METHOD FOR THE ISOLATION AND ESTIMATION OF GABA, GLUTAMATE AND GLUTAMINE FROM COMPLEX MIXTURES BY A COMBINATION OF ENZYMIC INTERCONVERSION AND FORMATION OF 1-DIMETHYLAMINONAPHTHALENE-5-SULPHONYLBUTYROLACTAM |
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| Authors: | R. H. C. Strang |
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| Affiliation: | Department of Biochemistry, University of Glasgow, Glasgow, Scotland |
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| Abstract: | Abstract— A method is presented which allows the isolation and estimation of GABA, glutamate and glutamine in extracts of nervous tissue. It depends on the enzymic conversion of the last two compounds to GABA, before isolating the GABA as DNS-γ-butyrolactam. The low polarity of this compound makes this possible without the use of chromatography. Furthermore, the specific radioactivities of these 3 compounds in a complex mixture may be found without elaborate separation procedures. Details are given of optimum reaction conditions, and comparisons made with other methods of estimation of these amino acids. |
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