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Ethanol production from starch by immobilized Aspergillus awamori and Saccharomyces pastorianus using cellulose carriers
Authors:Fujii N  Oki T  Sakurai A  Suye S  Sakakibara M
Affiliation:(1) Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Fukui 910-8507, Japan, JP
Abstract:A simultaneous saccharification and fermentation (SSF) process was investigated to produce ethanol using two kinds of cellulose carriers that were respectively suitable for immobilization of Aspergillus awamori and Saccharomyces pastorianus. The maximum ethanol concentration attained by the batch operation was 25.5 g l−1. Under suitable conditions, both cellulose carriers with immobilized cells could be reused efficiently for three cycles. The total amount of ethanol production was 66.0 g (per 1 l working volume) after the repeated operation. Ethanol productivity mainly depends on a saccharification process. There is a limit in durability in the repeated batch operation, and it is important to maintain high activity of the fungus in order to produce ethanol efficiently. Journal of Industrial Microbiology & Biotechnology (2001) 27, 52–57. Received 11 December 2000/ Accepted in revised form 02 June 2001
Keywords:: simultaneous saccharification and fermentation   fungus   production of ethanol, cellulose carrier   Aspergillus awamori   Saccharomyces pastorianus   repeated batch operation
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