Acyl migration during deacylation of phospholipids rich in docosahexaenoic acid (DHA): an enzymatic approach for evidence and study |
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Authors: | Laurent Poisson Marie Devos Stéphanie Godet Françoise Ergan Gaëlle Pencreac’h |
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Affiliation: | (1) Mer, Molécules, Santé-EA 2160, Université de Nantes, 44072 Nantes cedex 03, France;(2) Département Génie Biologique, Institut Universitaire de Technologie de Laval, 52 rue des Drs Calmette et Guérin, BP 2045, 53020 Laval Cedex 9, France |
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Abstract: | Non-enzymatic acyl migration could be counter-productive for the preparation of structured phospholipids with docosahexaenoic acid (DHA) at a designated position. Therefore enzymatic approaches have been developed to investigate acyl migration. First, acyl migration from sn-2 to sn-1 position has been set into relief by a three step enzymatic method using a typo-selective lipase, a phospholipase A2 and a non-selective lipase. The effect of reaction temperature on acyl migration from sn-2 to sn-1 was monitored: lowering the reaction temperature from 40 to 30°C allowed a reduction of DHA migration rate of 40%. Secondly, acyl migration from sn-1 to sn-2 position was negligible. This last result was obtained through the study of structured phosphatidylcholine selective deacylation using a phospholipase A2. |
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Keywords: | Acyl migration Docosahexaenoic acid Lipase Microalgal phospholipids Structured phospholipids |
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