Characterization of Neisseria meningitidis capsular polysaccharides containing sialic acid by pyrolysis mass spectrometry |
| |
Authors: | J Haverkamp H L Meuzelaar E C Beuvery P M Boonekamp R H Tiesjema |
| |
Affiliation: | 1. FOM-Institute for Atomic and Molecular Physics, Kruislaan 407, 1098 SJ Amsterdam, The Netherlands;2. National Institute for Public Health (RIV), A. van Leeuwenhoeklaan 9, 3721 MA Bilthoven, The Netherlands |
| |
Abstract: | The group B, C, W-135, and Y capsular polysaccharides of Neisseria meningitidis which contain sialic acid were differentiated by Curie-point pyrolysis low-voltage mass spectrometry. A large series of partially purified group B polysaccharide preparations obtained from pathogenic as well as nonpathogenic strains were analyzed by the same technique. It was shown that the carbohydrate structure of these group B polysaccharides appears to be the same throughout the whole series. Slight immunogenicity of some of the group B polysaccharide preparations tested is probably due to protein impurities. Automated pyrolysis mass spectrometry coupled with multivariate analysis of the spectral data by computer turns out to be a rapid method of characterizing microgram samples of large series of polysaccharide preparations. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|