The synthesis of radioactive 3-methylthiopropionate and other alkylthio fatty acids. |
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Authors: | R D Steele N J Benevenga |
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Affiliation: | 1. Department of Nutritional Sciences, The University of Wisconsin, Madison, Wisconsin 53706 USA;2. Department of Meat and Animal Science, The University of Wisconsin, Madison, Wisconsin 53706 USA |
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Abstract: | Aprocedure is described for the synthesis of radioactive 3-methylthiopropionate, a recently isolated metabolite of mammalian methionine metabolism. The method is a two-step synthesis whereby correspondingly labeled methionine is degraded by ninhydrin to 3-methylthiopropionaldehyde and then specifically oxidized to 3-methylthiopropionate without oxidation of the sulfur atom by the yeast enzyme, aldehyde dehydrogenase. Radiochemical purity of the isolated product was established by paper, thin-layer, and gas-liquid chromatography. This procedure is economical and readily applicable to the synthesis of other alkylthio fatty acids for the study of S-methylcysteine and ethionine metabolism and probably for the synthesis of radioactive intermediates of branched chain amino acids. |
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Keywords: | To whom requests for reprints should be sent. |
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