Preliminary x-ray crystallographic analysis of centrin from ciliate Euplotes octocarinatus |
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| Authors: | He Xiao-Jing Li Liu Hou Hai Zhao Yaqin Liang Aihua Rao Zihe |
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| Affiliation: | Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China. |
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| Abstract: | Centrins are four-EF-hand Ca(2+)-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 angstroms. The crystals belong to space group P2(1)2(1)2(1) and the unit-cell parameters are a=34.442 angstroms, b=48.954 angstroms, c=72.583 angstroms. |
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