Plant regeneration from protoplasts of Laminaria japonica Areschoug (Laminariales, Phaeophyceae) in a continuous-flow culture system |
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Authors: | T. Sawabe Y. Ezura H. Yamamoto |
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Affiliation: | (1) Laboratory of Microbiology, Department of Food Science and Technology, Faculty of Fisheries, Hokkaido University, 3-1-1 Minato-cho, Hakodate 041, Japan Fax no.: +81-138-40-5569 E-mail: sawabe@pop.fish.hokudai.ac.jp, JP |
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Abstract: | A continuous-flow culture system was developed for culturing Laminaria japonica protoplasts. Protoplasts were settled on 5-μm pore size nylon mesh fixed inside a 50-ml plastic syringe, and cultured in Provasoli's enriched seawater with iodine medium with a gentle upward flow generated by a peristaltic pump. In the culture system, 50% of the protoplasts regenerated their cell wall within 24 hours and almost all protoplasts regenerated a cell wall after 3 days culture. After cell wall regeneration, a number of cells divided and regenerated into sheet-shaped thalli. The thalli transferred to a tissue culture flask developed into sporophyte-like plantlets within 1 month. Plantlets then differentiated into blade, stipe, and holdfast, with a proper mucilage canal. Received: 21 April 1997 / Revision received: 27 June 1997 / Accepted: 5 July 1997 |
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Keywords: | Protoplast Laminaria japonica Plant regeneration Continuous-flow culture |
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