Synthesis of nascent prothrombin and albumin in a heterologous system using rat liver messenger RNA purified in oligo (dT)-cellulose. |
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| Authors: | N J Nardacci J P Jones A L Hall R E Olson |
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| Affiliation: | Department of Biochemistry St. Louis University School of Medicine St. Louis, MO 63104 USA |
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| Abstract: | Highly active m-RNA was prepared by phenol extraction of rat liver polysomes followed by oligo (dT)-cellulose chromatography. This m-RNA preparation stimulated total protein synthesis in rabbit reticulocyte lysates and in wheat germ extracts. Nascent prothrombin and albumin synthesized in the reticulocyte system programmed with this m-RNA were precipitated with specific antibodies and identified by their electrophoretic mobilities on SDS-acrylamide gels. |
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