Fermentation kinetics of Zymomonas mobilis near zero growth rate |
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Authors: | Jöbses I M Hiemstra H C Roels J A |
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Affiliation: | Laboratory of Bioengineering, Department of Chemistry and Chemical Engineering Delft University of Technology, Delft, The Netherlands. |
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Abstract: | The fermentation kinetics Zymomonas mobilis were studied near zero growth rate in fed-batch cultures and continuous cultures with complete cell recycle. The results show the ethanol enhances that specific substrate conversion rate under these conditions. The maximum achievable ethanol concentration in continuous cultures with cell recycle (66 g/L) was significantly lower than in fed-batch cultures (100 g/L). The results indicate that growth-rate-independent metabolism is not instantaneous and can lag behind steadily increasing ethanol concentrations in fed-batch fermentations. A model is proposed to account for this slow adaptation. |
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