| 乳酸菌食品级基因表达系统 |
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| 引用本文： | 张振中,陈秀珠,贾士芳,陈美玲,还连栋.乳酸菌食品级基因表达系统[J].生物工程学报,2002,18(4):516-520. |
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| 作者姓名： | 张振中 陈秀珠 贾士芳 陈美玲 还连栋 |
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| 作者单位： | 1. 中国科学院微生物研究所分子微生物学研究中心,北京,100080;中国科学院微生物研究所微生物资源前期开发国家重点实验室,北京,100080
2. 中国科学院微生物研究所分子微生物学研究中心,北京,100080 |
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| 基金项目： | 中国科学院“九五”重大项目 (No .KY95 J1 331)，中国科学院创新项目资助~~ |
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| 摘 要： | 酸菌是一类重要工业菌株。最近，乳酸菌遗传学和分子生物学的研究取得长足进步，导致发展了乳酸菌食品级基因表达系统。通过介绍乳酸菌食品级基因表达系统的基本要求、食品级选择性标记、食品级诱导物及该系统的研究进展，展示了乳酸菌食品级基因表达系统的建立对研究乳酸菌的基因表达调控和它的深层次的开发利用所具有的重要意义。
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| 关 键 词： | 乳酸菌， 食品级， 基因表达 |
| 文章编号： | 1000-3061(2002)04-0516-05 |
| 修稿时间： | 2001年10月18 |
Food-grade Gene Expression Systems for Lactic Acid Bacteria |
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| ZHANG Zhen-Zhong , CHEN Xiu-Zhu JIA Shi-Fang CHEN Mei-Ling HUAN Lian-Dong.Food-grade Gene Expression Systems for Lactic Acid Bacteria[J].Chinese Journal of Biotechnology,2002,18(4):516-520. |
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| Authors: | ZHANG Zhen-Zhong CHEN Xiu-Zhu JIA Shi-Fang CHEN Mei-Ling HUAN Lian-Dong |
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| Institution: | Research Center for Molecular Microbiology, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. |
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| Abstract: | Lactic acid bacteria (LAB) are important industrial microor ganism used in the production and preservation of foodstuffs. Recently, considerable advances have been made in the genetics and molecular biology of LAB. These have resulted in the construction of food-grade gene expression systems for these bacteria. This paper aims to review the essential features for food-grade systems, food-grade selection markers, food-grade controlled gene expression and food-grade inducible signaling molecule, and recent developments on food-grade cloning and expression systems for LAB.These gene expression systems have great potential for studies on gene expression and regulation in LAB and a variety of bioprocessing application in industrial fermentations. |
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| Keywords: | lactic acid bacteria food-grade gene expression |
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