Comparisons of procedures for determining ribosomal ribonucleic acid similarities |
| |
Authors: | J. L. Johnson Barbara Harich |
| |
Affiliation: | 1. Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, 24061, Blacksburg, Virginia, USA
|
| |
Abstract: | Ribosomal ribonucleic acid (rRNA) cistron similarities were determined forClostridium species with labeled preparations of 16S, 23S, or 16S plus 23S rRNA. Similarities were measured by membrane competition and by thermal stability experiments, and the results were correlated. Correlations were also made between 16S rRNA membrane competition homology values and similarities of 16S rRNA oligonucleotides. Very similar results were obtained whether 16S-or 23S-labeled rRNA was used. Clustering patterns were similar for homology values from hybridization experiments incubated at 50° or 60°C, as were the clustering patterns comparing homology values and rRNA hybrid thermal stability values. There was a linear correlation between rRNA homology values and 16S oligonucleotide SAB values (Tanner et al. [22]) for homology values above 20%, and SAB values above 0.45. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|