Determination of the strength of the cell walls of vegetative cells ofBacillus megaterium andBacillus stearothermophilus |
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| Authors: | J E Algie J A Lindsay I C Watt |
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| Institution: | 1. Division of Food Research, CSIRO, P.O. Box 52, 2113, North Ryde, New South Wales, Australia
2. Division of Textile Physics, CSIRO, 338 Blaxland Road, 2112, Ryde, New South Wales, Australia
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| Abstract: | The tensile strength of the cell walls ofBacillus megaterium andBacillus stearothermophilus was found to be about 2.4×107 N/m2. The internal pressure and water activity of the cells were 14 atm, 0.99 aw forB. megaterium and 28 atm, 0.98 aw forB. stearothermophilus. The greater strength ofB. stearothermophilus cells, considered as pressure vessels, restricts absorption of water by the protoplasm so that the water content on a dry weight basis is 3.4 g/g forB. megaterium cells in water but only 1.8 g/g forB. stearothermophilus. |
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