Proteins associated with mitochondrial DNA protect it against X-rays and hydrogen peroxide |
| |
Authors: | N A Gouliaeva E A Kuznetsova A I Gaziev |
| |
Institution: | (1) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia |
| |
Abstract: | The high susceptibility of mitochondrial DNA to reactive oxygen species and other damaging agents has been supposed to result from the absence of histones. Here we show that DNA-binding proteins of mitochondrial nucleoids can shield mtDNA from X-ray radiation and hydrogen peroxide just as nuclear histones do. Mitochondria, mitochondrial nucleoid proteins, and histones were isolated from mouse liver and assessed for mtDNA protection by the yield of PCR products. In vitro, mtDNA in complex either with nucleoid proteins or with nuclear histones proved to be much less damaged than naked mtDNA, with little difference in protective efficacy. Most probably, in mitochondria the nucleoid proteins also protect mtDNA against reactive oxygen species and thus attenuate the oxidative damage. |
| |
Keywords: | mitochondrial DNA DNA-binding proteins histones oxidative damage |
本文献已被 SpringerLink 等数据库收录! |
|