Differentiation ofPetunia hybrida tissues transformed byAgrobacterium rhizogenes andAgrobacterium tumefaciens |
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Authors: | M. Ondřej Růzena Bísková |
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Affiliation: | (1) Institute of Experimental Botany, Czechoslovak Academy of Sciences, 370 05 České Budějovice, Na sádkách 702, Czechoslovakia |
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Abstract: | Petunia hybrida plants were inoculated with differentAgrobacterium rhizogenes andA. tumefaciens strains and developed tumors were further cultivatedin vitro. Transformed flowering plants differentiated from tumors induced byA. rhizogenes strains 8196 and TRIOL Transformed but non-rooted plants developed also from tumors incited byA. tumefaciens T37. Cultures of roots transformed byA. rhizogenes strain 15834 did not show increased incidence of chromosomal aberrations in anaphases in comparison with untransformecl control. Permanent growth of isolated untransformedPetunia roots was not induced by addition of IAA into the medium. |
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