Behavior of primary and serially transplanted estrogen-dependent renal carcinoma cells in monolayer and in collagen gel culture |
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Authors: | Deanna J Talley William A Roy Jonathan J Li |
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Institution: | (1) Division of Biochemistry, Department of Basic Sciences, Marquette University School of Dentistry, 604 North 16th Street, 53233 Milwaukee, Wisconsin;(2) Division of Anatomy, Department of Basic Sciences, Marquette University School of Dentistry, 604 North 16th Street, 53233 Milwaukee, Wisconsin;(3) Medical Research Laboratories, Veterans Administration Medical Center, and Department of Urologic Surgery, University of Minnesota Medical School, 55417 Minneapolis, Minnesota |
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Abstract: | Summary When estrogen is present in culture medium, enzyme-dissociated cells from estrogen-induced primary renal tumors in Syrian
hamsters and from 1st to 4th serially transplanted carcinomas in monolayer culture contain progesterone receptor levels that
are similar and comparable to those in tumors in vivo (i.e., 2 pmol/3×106 cells). Despite the similarity of receptor levels in cultured cells isolated from primary and transplanted tumors, the ability
of cells to be maintained in culture differs considerably from one tumor stage to another. When cultured as monolayers in
plastic flasks, isolated cells from primary tumors exhibit a marked decline in cell number after 4 to 6 d in culture. On the
other hand, monolayer-cultured cells from first and second transplantation tumors remain essentially constant in cell number
over a 2 wk culture period and cells from third transplantation tumors undergo a two- to threefold increase in cell number
during 2 wk in culture.
When primary tumor cells are cultured in collagen gels, the decline in cell number over a 2 wk culture period is prevented
and progesterone receptor levels remain elevated. Cells cultured from first transplantation tumors exhibit a delayed decline
in cell number beginning after 2 wk in monolayer culture. The decline in cell number in monolayer culture, like that for cells
from primary tumors, can be prevented by culturing cells from first transplantation tumors in collagen gels. Neither cells
from primary nor first transplantation tumors exhibit significant increases in cell number in collagen gels. Increasing the
serum concentration of growth medium to 30% does not stimulate growth of cells under these conditions. Cells isolated from
fourth transplantation tumors undergo a fourfold increase in cell number over a 1 month culture period whether cells are cultured
as monolayers or in collagen gels.
This investigation was supported by Grant CA 22008 awarded by the National Cancer Institute, Department of Health, Education
and Welfare, and by institutional research funds from Marquette University. |
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Keywords: | epithelial culture renal tumor estrogen collagen |
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