A simple autoradiographic technique for studying diffusion of water into seeds |
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Authors: | Jackson G M Varriano-Marston E |
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Affiliation: | Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506. |
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Abstract: | Visual observations on rates and modes of water penetration into black bean seeds and into wheat and sorghum kernels during conditioning were accomplished by an autoradiographic procedure that eliminates a freezing microtome and liquid and stripping film emulsions. Seeds soaked in tritiated H2O were hand sectioned before freeze-quenching in liquid N2 and subsequent block autoradiography on nuclear medicine film. |
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