首页 | 本学科首页   官方微博 | 高级检索  
   检索      


A characteristic electrophoretic pattern of cytosolic polypeptides from hepatocyte nodules generated during liver carcinogenesis in several models
Authors:L C Eriksson  R N Sharma  M W Roomi  R K Ho  E Farber  R K Murray
Institution:1. Clinical Neuropharmacology Branch, NIMH; National Institutes of Health, Bethesda, Md. 20205 USA;2. Laboratory of Chemistry, NHBLI; National Institutes of Health, Bethesda, Md. 20205 USA;3. Laboratory of Clinical Science, NIMH; National Institutes of Health, Bethesda, Md. 20205 USA;4. Laboratory of Chemistry, NIADDK; National Institutes of Health, Bethesda, Md. 20205 USA;5. Laboratory of Bio-organic Chemistry, NIADDK; National Institutes of Health, Bethesda, Md. 20205 USA
Abstract:The cytosolic polypeptides of hepatocyte nodules in six models of liver carcinogenesis were analysed by SDS-polyacrylamide gel electrophoresis and their patterns compared with these of control and variously treated livers. The amount of a polypeptide of Mr 21,000 was about tenfold elevated in the cytosol of five of the six types of nodules and moderately elevated in the sixth. Certain other polypeptides, particularly one of Mr 26,000, also varied in amount, so that all of the nodules analysed could be distinguished from liver by their electrophoretic patterns. Some possible identities of the two polypeptides are discussed. Their study may have mechanistic as well as diagnostic importance.
Keywords:CEI  chronic enzyme induction  CMD  choline-methionine deficient  2-AAF  2-acetylaminofluorene  PMSF  phenylmethyl-sulfonylfluoride  SDS-PAGE  sodium dodecyl sulfate-polyacrylamide gel electrophoresis  GSH  glutathione  DEN  diethylnitrosamine  PB  phenobarbital  3-MC  3-methylcholanthrene  3′-Me-DAB  3′-methyl-4-dimethylaminoazobenzene  DMH  1  2-dimethylhydrazine  GGT  γ-glutamyl transpeptidase
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号