Estimation of tyrosine by the attenuation of 4-iodophenol enhanced chemiluminescence |
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Authors: | Thomas E. G. Candy David Mantle Peter Jones |
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Abstract: | Tyrosine markedly attenuates the chemiluminescence output intensity from the 4-iodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4-iodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic acid. |
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Keywords: | Enhanced chemiluminescence tyrosine analysis protein catabolism |
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