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基于克隆文库法研究古尔班通古特沙漠蓝藻多样性
引用本文:刘乐汉,吕 杰,马 媛,吕光辉. 基于克隆文库法研究古尔班通古特沙漠蓝藻多样性[J]. 西北植物学报, 2021, 41(7): 1127-1136
作者姓名:刘乐汉  吕 杰  马 媛  吕光辉
作者单位:(1 新疆大学 资源与环境科学学院,乌鲁木齐 830046;2 新疆大学 生命科学与技术学院,乌鲁木齐 830046;3 绿洲生态教育部重点实验室,乌鲁木齐 830046)
基金项目:国家自然科学基金(31860149);
摘    要:该研究采用克隆文库法研究古尔班通古特沙漠藻类生物结皮中蓝藻多样性及分布.在古尔班通古特沙漠不同区域采集10份藻类结皮代表性土样,分别构建古尔班通古特沙漠蓝藻16S rRNA和psbA基因克隆文库,并进行系统发育分析,对蓝藻多样性和丰度与环境因子进行关联分析,研究蓝藻分布特点及影响因子.结果 显示:(1)16S rRNA...

关 键 词:生物土壤结皮  古尔班通古特沙漠  蓝藻多样性  克隆文库

Cyanobacterial Diversity of Gurbantunggut Desert Based on Clone Library, Xinjiang, China
Abstract:The goal of this study was to research the cyanobacterial diversity and distribution in algal crusts of Gurbantunggut Desert by the clone library method. In this paper, 10 representative algal crust soil samples were collected in different regions of the Gurbantunggut Desert, and the cyanobacterial 16S rRNA and psbA gene clone libraries were constructed and performed phylogenetic analysis. Moreover, the correlation of cyanobacterial diversity and abundance with environmental factors was analyzed to acquire the distribution characteristics and influencing factors of cyanobacteria. The results showed: (1) the cyanobacterial 16S rRNA gene belonged to 10 genera of 6 families (accounted for 94.85% of the total library) and an uncultured cyanobacteria by phylogenetic analysis. Oscillatoria and Microcoleus accounted for 42.54% and 37.16% of the library respectively, which were the dominant genus of cyanobacteria in Gurbantunggut desert. Only 4 genera of 4 families cyanobacteria were identified with the psbA gene by phylogenetic analysis, but the dominant genera were consistent with the former results. (2) The species of cyanobacteria in algal crusts were different from each other in the 10 sample sites, but Oscillatoria and Microcoleus were found in each sample site, which proved that they were the dominant genera in algal crusts of Gurbantunggut Desert. In addition, there were more cyanobacteria species in Gur2 and Gur17, and fewer cyanobacteria species in Gur3, Gur5 and Gur9. However, the relative geographical locations of Gur2, Gur3, Gur5 and Gur17 were relatively close, indicating that geographical location was not the main factor affecting the distribution of cyanobacteria. (3) Redundancy analysis results showed that microbial biomass nitrogen (MBN) and soil organic carbon (SOC) have the greatest impact on the diversity of cyanobacteria, followed by nitrate nitrogen (NO-3 N), microbial biomass carbon (MBC). Total phosphorus (TP) and total potassium (TK) have the least impact. The research showed that the cyanobacteria diversity and physicochemical properties of the soil in different regions of the Gurbantunggut Desert are spatially heterogeneous. The algae crusts in the middle, south of the desert are the most nutritious and the diversity of cyanobacteria is higher, while the algae crusts in the east, west are poor in nutrition, and the cyanobacteria richness and diversity are lower.
Keywords:biological soil crusts   Gurbantunggut Desert   diversity of Cyanobacteria   clone library
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