| 大豆11S球蛋白Gy5(A3B4)的基因克隆和序列分析 |
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| 引用本文： | 陈三凤.大豆11S球蛋白Gy5(A3B4)的基因克隆和序列分析[J].生物工程学报,2000,16(2):215-217. |
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| 作者姓名： | 陈三凤 |
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| 作者单位： | 深泽亲房日本国立食品研究所筑波日本中国农业大学生物学院北京 100094 |
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| 摘 要： | 大豆11Ｓ球蛋白(Ｇｌｙｃｉｎｉｎ)是大豆种子的主要贮藏蛋白,分子量为360ｋＤ,由6对相同的蛋白亚基(每对亚基的分子量约60ｋＤ)构成。每对亚基又是由一个酸性Ａ肽(35～45ｋＤ)和一个碱性Ｂ肽(22ｋＤ)通过二硫键连接而成。Ａ肽和Ｂ肽源自同一个基因,即首先由一个大的ｍＲ?..
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| 关 键 词： | 大豆球蛋白 Gy5（A3B4）基因 外显子 DNA 序列分析 |
| 文章编号： | 1000-3061(2000)02-0215-03 |
| 修稿时间： | 1999年3月9日 |
Molecular Cloning and Nucleotide Sequence of Soybean Glycinin Gene Gy5(A3B4) |
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| CHEN San,Feng.Molecular Cloning and Nucleotide Sequence of Soybean Glycinin Gene Gy5(A3B4)[J].Chinese Journal of Biotechnology,2000,16(2):215-217. |
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| Authors: | CHEN San Feng |
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| Institution: | Dept. of Microbiology, China Agricultural University, Beijing. |
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| Abstract: | The glycinin gene family encoding the glycinin subunits in soybean plants is composed of at least 5 gene members, i.e :Gy1-Gy5.A genomic clone containing the Gy5 gene from a genomic library of cv.Williams was isolated by using Gy5 cDNA probe.The complete nucleotide sequence of this gene has been determined.It is 2819 bp long consisting of four exons and three introns.These exons and introns are as follows:exonl(292bp),intronl(358bp),exon2(263bp),intron2(425bp),exon3(645bp),intron3(485bp),exon4(351bp).The gene encodes 517 amino asids.This is the first time to report the complete Gy5 gene sequence from a genomic library.The accession number in the DDBJ database is AB003680. |
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| Keywords: | Glycinin Gy5(A 3B 4)gene exon intron DNA sequence |
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