Increase in superoxide production by heat-shocked cells of Neurospora crassa, demonstrated by a fluorometric assay. |
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Authors: | W S Lin M Kapoor |
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Institution: | Department of Biological Sciences, University of Calgary, Alberta, Canada. |
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Abstract: | 1. Increase in superoxide production by heat-shocked cells of Neurospora crassa was demonstrated by a fluorometric assay. 2. A sensitive fluorometric assay for the estimation of superoxide anion radical--based on the liberation of 4-methylumbelliferone from 4-methyl-beta-D-umbelliferyl glucopyranoside--is described. 3. Using this system the level of superoxide in the medium of heat-shocked Neurospora crassa cells was found to be consistently higher, in comparison with that of non-shocked cells, cultured at the normal growth temperature of 28 degrees C. 4. Addition of superoxide dismutase to the culture media suppressed the production of 4-methylumbelliferone. |
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