Obtaining a High Percentage of Explants with Negative Serological Reactions against viruses by Combining Potato Meristem Culture with Antiphytoviral Chemotherapy |
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| Authors: | Dipl agr S Borissenko Prof Dr sc G Schuster Doc Dr W Schmygla |
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| Institution: | Timirjasew -Academy of Agricultural Sciences, Chair of Phytopathology, Lietwennitschnaja alleja, d 2, utsch. korp. 12, 127550 Moscow 7-550 (Soviet Union).;Karl-Marx-Universität, Sektion Biowisscnschaften, Wissenschaftsbereich Pflanzenphysiologie und Mikrobiologie, Talstraße 33, 7010 Leipzig (German Democratic Republic). |
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| Abstract: | A high percentage of plantlets, in which PVX, PVM, and PVS were no longer detectable by precipitin tests was obtained from 100% virus-infected stocks of the potato varieties Rosa and Priobskij rannija by combining meristem (tip) culture with antiphytoviral chemotherapy. When 2,4 - dioxohexahydro 1,3,5-triazinc (DHT) was added to the nutrient media, no virus was detectable serologically in 72% of the corresponding explants of the variety Rosa and in 66% of that of the variety Priobskij rannija, respectively. Contrary to this, all untreated control explants proved virusinfected. Treatment with 2-thiouracil resulted in 46.6%, with cyanoguanidine in 28.6% of plantlets, in which no virus was detectable serologically. |
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