氯氰菊酯降解菌草酸青霉SSCL-5分离鉴定及降解特性

为解决土壤中残留的菊酯类农药问题,通过富集筛选法从70余份土壤中获得一株高效分解利用菊酯类农药的微生物菌株SSCL-5,该菌株可在含1 000 mg/L的氯氰菊酯无机盐培养基中正常生长。经形态学及ITS测序鉴定,确定该菌为草酸青霉(Penicillium oxalicum),对多种高浓度菊酯类农药耐受。经紫外分光光度法及HPLC验证该菌株草酸青霉SSCL-5在无机盐培养基、28℃、180 r/min摇瓶培养24 h的条件下,对400 mg/L氯氰菊酯的降解率为97%。土壤室内试验证明该菌在土壤中,温度20-34℃、水分含量保藏40%-60%条件下,30 d可将土壤中400 mg/L的氯氰菊酯降解67.6%。

Isolation and Identification of Penicillium oxalicum SSCL-5 Degrading Cypermethrin and Its Biodegradation

To find a solution of removing pyrethroid pesticide residues in soil,enrichment and screening methods were used,and a microbial strain,which efficiently decomposed and utilized pyrethroid-like pesticides,was isolated from over 70 soil samples,and named as SSL-5.The strain grew normally in the mineral medium containing 1000 mg/L cypermethrin.According to the results of morphology,ITS Sequencing analysis,the strain SSCL-5 was identified as Penicillium oxalicum,and tolerant to other several pyrethroid-like pesticides in high concentration.Degradation rate of cypermethrin(400 mg/L)by this SSL-5 in the mineral medium at 28℃and 180 r/min and culturing 24 h was verified to be 97%with HPLC and UV spectrophotometry.Laboratory soil tests proved that cypermethrin in soil(400 mg/L)was degraded by 67.6%in 30 d while temperature in 20℃-34℃and moisture content in 40%-60%.