Biosynthesis of medium chain length poly(3-hydroxyalkanoates) (mcl PHAs) from cosmetic co-products by Pseudomonas raguenesii sp. nov., isolated from Tetiaroa, French Polynesia |
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Authors: | C Simon-Colin K Alain G Raguns S Schmitt N Kervarec C Gouin P Crassous B Costa JG Guezennec |
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Institution: | C. Simon-Colin, K. Alain, G. Raguénès, S. Schmitt, N. Kervarec, C. Gouin, P. Crassous, B. Costa,J.G. Guezennec |
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Abstract: | A new bacterium, designated as strain TE9 was isolated from a microbial mat in French Polynesia and was studied for its ability to synthesize medium chain length poly-β-hydroxyalkanoates (mcl PHAs) during cultivation on cosmetics co-products. The composition of PHAs was analysed by coupled gas chromatography mass spectroscopy (GC/MS), nuclear magnetic resonance (NMR) and Fourier Transform InfraRed (FTIR) spectroscopy. PHAs were composed of C6–C14 3-hydroxyacids monomers, with a predominance of 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD) and 3-hydroxydodecanoate (3HDD). Differential scanning calorimetry (DSC) experiments allowed the characterization of elastomeric materials with a melting point Tm near 50 °C, enthalpy of fusion ΔHm from 27 to 32 J/g, and glass transition temperature Tg of −43 °C. Molecular weights ranged from 175,000 to 358,000 g/mol. On the basis of the phenotypical features and genotypic investigations, strain TE9 was assigned to the Pseudomonas genus and the name of Pseudomonas raguenesii sp. nov. is proposed. |
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Keywords: | Microbial mat Pseudomonas mcl PHAs Polyhydroxyalkanoates Co-products |
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