基因枪法转化小麦的金粉用量及转基因植株表型特征分析

研究了不同金粉用量对小麦幼胚瞬间及稳定转化频率的影响，结果表明此实验系统的金粉用量以每枪500μg金粉为佳。对获得的T

Gold Particle Amount perBombardment of Wheat Transformation by Biolistic Method and Phenotypic Character Analysisof Transgenic Plants and Their Progenes

Effects of gold particle amount per bombardment (30, 100, 250,500 μg) on transient and stable transformation of immature wheat embryos, were studied. And 500 μg gold particle per bombardment is superior to others both in GUS transient expression and transgenic plant production (Fig. 2). PCR and Southern analyses of T0 and Tt plants confirmed the integration of foreign bar gene fragments into wheat genomes (Figs. 5～7). Tt plants smeared with L-PPT 200 mg/L showed a lowlevel of resistance to this herbicide (Plate Ⅰd). The comparison of phenotypic character of To, T1, T2 and their control plants showed that the phenotype abnormality of To plants is only a temporary phenomenon due to physiological and cultivated conditions, and the plants will recover from this abnormality partially or fully in the following progenes (Table 2 and Plate Ⅰ-e).