Improvement of the catharanthine productivity in hairy root cultures ofCatharanthus roseus by using monosaccharides as a carbon source |
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| Authors: | K H Jung S S Kwak S W Kim H Lee C Y Choi J R Liu |
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| Institution: | (1) Plant Cell Biology Laboratory, Genetic Engineering Research Institute, Korea Institute of Science and Technology, P.O. Box 17, 305-606 Taedok Science Town, Taejon, Korea;(2) Biochemical Engineering Major, Seoul National University, 151-742 Seoul, Korea;(3) Department of Chemical Engineering, KAIST, Kusung-dong, Yusung-gu, Taejon, Korea |
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| Abstract: | Summary Sucrose, glucose, and fructose as carbon sources in culture medium were assessed in hairy root cultures ofCatharanthus roseus. The cultures preferentially consumed sucrose, resulting in about 40% (dry wt) higher growth rate. However, fructose enhanced the cathranthine yield about two-fold. The elevated yield was not seemingly ascribed to the higher osmolarity per unit weight of fructose than sucrose. A two stage culture using sucrose (1st) and fructose (2nd) improved volumetric yields of catharanthine about two-fold, i.e. 41 mg/l. |
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