Sivb 2003 Congress Symposium Proceeding: Mutation- and Transposon-Based Approaches for the Identification of Genes for Pre-Harvest Sprouting in Wheat

Summary This article reviews techniques for gene identification and cloning in allohexaploid bread wheat (Triticum aestivum L.). Gene identification and cloning in wheat are complicated by the large size and high redundancy of the genome. Both classical mutagenesis and transposon tagging are important tools for the study of grain dormancy and plant hormone signaling in wheat. While classical mutagenesis can be used to identify wheat mutants with altered hormone sensitivity, it can be difficult to clone the corresponding genes. We review the techniques available for gene identification in wheat, and propose that transposon-based activation tagging will be an important tool for wheat genetics.