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| 丙型肝炎病毒准种血清学检测技术的建立 | |
| 引用本文： | 张向颖,修冰水,张贺秋,祁自柏.丙型肝炎病毒准种血清学检测技术的建立[J].生物技术通讯,2010,21(2):240-243. |
| 作者姓名： | 张向颖 修冰水 张贺秋 祁自柏 |
| 作者单位： | 1. 中国药品生物制品检定所,北京,100050;军事医学科学院,基础医学研究所,北京,100850 2. 军事医学科学院,基础医学研究所,北京,100850 3. 中国药品生物制品检定所,北京,100050 |
| 基金项目： | "十一五"国家科技重大专项，国家高技术研究发展计划目标导向课题，国家自然科学基金 |
| 摘 要： | 目的:建立一种以血清学为基础的丙型肝炎病毒(HCV)准种检测技术。方法:自20份HCV血清中各挑选30个克隆进行测序比较,分析HCV准种的复杂程度;以HCV准种代表性抗原组合制备免疫芯片,用血清学检测技术分析上述20份HCV血清中的准种变异程度;比较两种方法之间的检出灵敏度和相关性。结果:测序法检出灵敏度为70.0%,血清学检测法检出灵敏度为95.0%,后者显著高于前者(P0.05);两种方法检测结果的相关性为74.7%(P0.01)。结论:血清学检测技术操作简单,且能够反映丙型肝炎患者的HCV准种变异程度,适于临床推广。 |
| 关 键 词： | 丙型肝炎病毒 准种 第一高变区 血清学检测 克隆测序 |
The Serology Detection for Hepatitis C Virus Quasispecies |
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| ZHANG Xiang-Ying,XIU Bing-Shu,ZHANG He-Qiu,QI Zi-Bai.The Serology Detection for Hepatitis C Virus Quasispecies[J].Letters in Biotechnology,2010,21(2):240-243. | |
| Authors: | ZHANG Xiang-Ying XIU Bing-Shu ZHANG He-Qiu QI Zi-Bai |
| Institution: | ZHANG Xiang-Ying1,2,XIU Bing-Shui2,ZHANG He-Qiu2,QI Zi-Bai1 1.National Institute for Control of Pharmaceutical , Biological Products,Beijing 100050,2.Institute of Basic Medicine Science,Academy of Military Medical Sciences,Beijing 100850,China |
| Abstract: | Objective:To establish the technology for hypervarible region 1(HVR1) of hepatitis C virus(HCV) by serology detection and compare its results to clone sequencing.Methods:RT-PCR sequence analysis and serology detection were used to find out the variation of HVR1,then their relativity and sensitivity were compared.Results:The sensitivity of the sequence analysis was 70.0%,compared to 95.0% in the serology detection,which was more sensitive obviously(P<0.05).The relativity between the two methods was 75%(P<0.0... |
| Keywords: | hepatitis C virus quasispecies hypervarible region 1 serology detection clone sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |